The molar extinction coefficient (ε) for DyLight 488 is 70,000 cm-1M-1. To request additional information and datasets for DyLight 488, please contact info@aatbio.com. Unless otherwise specified, molar extinction coefficient (ε) values are typically measured in ddH2O or PBS.

ATTO 488. 29. ATTO 495. 30. 500 nm - 600 nm. ATTO 520. 31. ATTO 532. 32 its rate is proportional to the extinction coefficient of the acceptor dye in the.

av A SVANSSON · Citerat av 46 — 3 a)0we may derive an exchange coefficient of 3 x 10 km /year Munthe-Kaas, H., 1968: Surface pollution and light extinction in the vol. 17, 476-488. Two-photon absorption in gapped bilayer graphene with a tunable chemical potential2016Ingår i: Journal of Physics: Condensed Matter, ISSN 0953-8984, 488-493Artikel i tidskrift (Refereegranskat). Abstract [en]. Hatchery-reared salmon smolt used for supplementary stocking often display poor migration behavior av G Pleijel · 1954 · Citerat av 44 — Since the attenuation of radiation on account of absorption and extinction is different for The ratio between the illumination and the heat radiation from the same radiation VII . 780 770 765 748 720 682 617 488 Krarup, Niels H. Sammenlignende underse>gelse af trre- og stålstilladser til husbygning.

Af 488 extinction coefficient

AFDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AFDye 488 has green fluorescence that is pH independent from pH 4 to pH 10 with excitation ideally suited for the 488 nm laser line. EXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefﬁcient of an oligo by either summing up the extinction coefﬁcients of the individual bases times their number of occurrences. Or use a formula that takes into account nearest neighbor effects. An algorithm for this calculation can be found on the web.

Extinction coefficient: 40,000 Molecular weight: ~429 Excitation laser line: 405 nm Replaces: Pacific Green®, BD Horizon™ V500, Krome Orange™ CF®440 Abs/Em Maxima: 440/515 nm Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430

The correlation between the coefficients provides information on the extinction wavelength dependency. 2002-09-01 2015-03-09 Extinction Coefficient (εmax) 7.3 x10 4 Fluorescence Quantum yield (Φf) 0 .92 Fluorescence Decay Time (τfl) 5.0 ns Brightness 67.2 Laser Line 488 nm Filter Set FITC Please Note: All products are for in vitro research use only and are not intended for use in humans or animals. Extinction coefficient: 40,000 Molecular weight: ~429 Excitation laser line: 405 nm Replaces: Pacific Green®, BD Horizon™ V500, Krome Orange™ CF®440 Abs/Em Maxima: 440/515 nm Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430 Extinction coefficient (€) Ex Filter Em Filter Mirror (cut off) Azurite 384 450 26,200 380/20 460/40 435 EBFP 383 445 29,000 380/20 460/40 435 GFP-S65T 488 509 56,000 485/20 516/20 510 mWasabi 493 509 70,000 485/20 516/20 510 Stemmer 395 509 27,000 395/25 508/20 435 sfGFP 203,000 = molar extinction coefficient ( ε) of a IgG at 280 nm [cm-1.

Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling

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extinction coefficient in the calculation yields an expression of concentration in terms of . molarity: A / ε. molar = molar concentration . However, many sources, including the reference cited above, do not provide molar extinction coefficients.
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A large number of rhodamine dyes, including Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 568, ATTO488, ATTO532, and ATTO565, were found to form stable radical anions upon irradiation in the presence of thiol compounds such as β-mercaptoethylamine (MEA). 67 Radical anions are formed by reduction of the triplet state in a reaction that also results in the formation of a thiyl radical (Fig. 1.10). AFDye™ 488 Alkyne (Alexa Fluor® 488 Alkyne equivalent) is a bright, green-fluorescent, alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). A large number of rhodamine dyes, including Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 568, ATTO488, ATTO532, and ATTO565, were found to form stable radical anions upon irradiation in the presence of thiol compounds such as β-mercaptoethylamine (MEA). 67 Radical anions are formed by reduction of the triplet state in a reaction that also results in the formation of a thiyl radical (Fig. 1.10).
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Azide-fluor 488 ≥90% (HPLC) Synonym: Rhodamine 110-azide Empirical Formula (Hill Notation) C 29 H 30 N 6 O 7. Molecular Weight 574.58 . NACRES NA.22 . SDS

† Extinction coefficient at Molar Extinction Coefficients vs. Absorbances for 1% Solutions . Application of a .

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Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0

Select fluorophores with high extinction coefficients ( ε ) The commonly used and very bright Alexa Fluor 488 fluorophore for example has a high quantum View and buy high quality TFAX 488, TFP. Extinction Coefficient (ε), 73,000 M- 1cm-1 Alternative For, Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2 Western blot Alexa Fluor® conjugated secondaries optimized for high quality Alexa Fluor®, Absorption Max (nm), Emission Max (nm), Extinction Coefficient. 5 Apr 2005 bance of the protein (protein extinction coefficients determined by various means) at 280 nm and the absor- bance of Alexa-Fluor 488 (molar Extinction Coefficient [Alexa Fluor 488]. The molar extinction coefficient (ε) for Alexa Fluor 488 is 73,000 cm-1M 22 May 2017 For example, DyLightTM 350 has an extinction coefficient value of 15,000 (dim) As an example, FITC cannot be used with Alexa Fluor® 488. proportional to the product of the extinction coefficient and the quantum yield. Note that the 488-nanometer argon-ion laser spectral line excites Alexa Fluor Extinction coefficient: 70,000. Molecular weight: ~914. Excitation laser line: 488 nm.